TNF-alpha (Tumor Necrosis Factor alpha) Antibody

• Catalog Number: orb2319977
• Category: Antibodies
• Description: TNF-alpha (Tumor Necrosis Factor alpha) Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TNF706
• Tested applications: FC, IF, IHC
• Reactivity: Canine, Feline, Human, Mouse, Rabbit, Rat, Zebrafish
• Isotype: IgM
• Immunogen: Recombinant N-terminal fragment of human TNF alpha
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-paraffin) (2-4ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein L. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 17kDa
• Target: TNF
• Entrez: 7124
• UniProt ID: P01375
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: APC1, Cachectin, Differentiation inducing factor (
• Note: For research use only
• Application notes: Positive Control: HePG2, HeLa, HL-60, or A431 cells. Macrophages in lymph node or tonsil (IHC).
• Expiration Date: 12 months from date of receipt.