TNF alpha Antibody

• Catalog Number: orb48033
• Category: Antibodies
• Description: TNF alpha Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Mouse
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived mouse TNF alpha recombinant protein (Position: L80-L235).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat ELISA , 0.1-0.5μg/ml, Mouse, -Western blot, 0.1-0.5μg/ml, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 25896 MW
• UniProt ID: P06804
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Tumor necrosis factor;Cachectin;TNF-alpha;Tumor ne
• Note: For research use only
• Application notes: By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of TNF alpha using anti-TNF alpha antibody.Lane 1: Recombinant Mouse TNFα Protein 0.5ng.