TNF alpha antibody

• Catalog Number: orb396138
• Category: Antibodies
• Description: Mouse monoclonal antibody to TNF alpha.
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: D2E7 (Adalimumab)
• Tested applications: Blocking, ELISA, IF, IHC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: This antibody was generated by affinity maturation from the 2SD4 scFv, which in turn was selected on human TNF alpha by guided phage-display technology, using the murine anti-hTNF alpha antibody Mab32 as a template (c.f. also US Patent 6090382).
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: TNF alpha
• UniProt ID: P01375
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Tumor necrosis factor alpha
• Note: For research use only
• Application notes: Adalimumab binds specifically to TNF and neutralizes the biological function of TNF by blocking its interaction with the p55 and p75 cell surface TNF receptors. Adalimumab, unlike Infliximab (Ab00146), was shown to be cross-reactive with mouse TNF alpha (Arnoult 2017, PMID: 28584008).
• Expiration Date: 12 months from date of receipt.

References

Jespers, L. S. et al. Guiding the selection of human antibodies from phage display repertoires to a single epitope of an antigen Biotechnology (N Y), 12, 899-903 (1994)

PubMed: 7521646