TNF alpha Antibody

• Catalog Number: orb180708
• Category: Antibodies
• Description: TNF alpha Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived rat TNF alpha recombinant protein (Position: D89-L235). Rat TNF alpha shares 95% amino acid (aa) sequence identity with mouse TNF alpha.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 25806 MW
• UniProt ID: P16599
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Tumor necrosis factor;Cachectin;TNF-alpha;Tumor ne
• Note: For research use only
• Application notes: WB: The detection limit for TNF alpha is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of TNF alpha using anti-TNF alpha antibody.Lane 1:PC-12 Cell;2:Rat Spleen Tissue;3:Rat Brain Tissue;4:Rat Kidney Tissue;5:Rat Liver Tissue.