TNF alpha Antibody

• Catalog Number: orb1294347
• Category: Antibodies
• Description: Rabbit polyclonal antibody to TNF alpha
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit-IgG
• Immunogen: Recombinant fragment corresponding to Human TNF alpha.
• Dilution range: Western Blot 1:500-1:1500 Immunofluorescence 1:200-1:300 Immunohistochemistry (Paraffin) 1:100-1:200
• Conjugation: Unconjugated
• Target: TNF alpha
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-TNF alpha antibody at 1/700 dilution, Lysates at 50 μg per lane, This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294347 overnight at 4°C.

Anti-TNF alpha antibody at 1/700 dilution, Lysates at 50 μg per lane, This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294347 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human Liver cancer tissue labeling TNF alpha with orb1294347 at 1/150.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294347 Anti-TNF alpha antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.