TJP1 Antibody

• Catalog Number: orb1247273
• Category: Antibodies
• Description: TJP1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-PKPTSQNQFSEHDKT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 170kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 187kDa according to NP_783297.2). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Expression of the protein seen in the cell junctions of A431 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TJP1
• UniProt ID: Q07157
• NCBI: NP_003248.3, NP_783297.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: TJP1, tight junction protein 1 (zona occludens 1),
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 170kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 187kDa according to NP_783297.2). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Expression of the protein seen in the cell junctions of A431 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247273 (0.3 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247273 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cell junction staining.