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Catalog Number | orb1474493 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to Survivin |
Species/Host | Rabbit |
Clonality | Monoclonal |
Tested applications | IF, IP, WB |
Reactivity | Human |
Immunogen | Recombinant protein of human Survivin |
Dilution range | WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50) |
Form/Appearance | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin. |
Conjugation | Unconjugated |
Target | BIRC5 |
Entrez | 332 |
UniProt ID | O15392 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin. |
Alternative names | API4; IAP4; Baculoviral IAP repeat-containing prot Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Survivin expression in Jurkat (A) whole cell lysates. (Predicted band size: 16 kD; Observed band size: 16 kD)
Immunofluorescent analysis of Survivin staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
ELISA, ICC, IF, WB | |
Bovine, Mouse, Porcine, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, IHC-P, WB | |
Bovine, Porcine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IHC-P, WB | |
Bovine, Canine, Equine, Guinea pig, Porcine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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