Survivin/BIRC5 Antibody

Catalog Number: orb18627

Catalog Number	orb18627
Category	Antibodies
Description	Survivin/BIRC5 Antibody
Species/Host	Rabbit
Clonality	Polyclonal
Tested applications	ELISA, IHC, WB
Reactivity	Human, Mouse, Rat
Isotype	Rabbit IgG
Immunogen	E. coli-derived human Survivin recombinant protein (Position: M1-D142). Human Survivin shares 84.3% and 83% amino acid (aa) sequence identity with mouse and rat Survivin, respectively.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Dilution range	Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat ELISA , 0.1-0.5μg/ml, Human, -
Form/Appearance	Lyophilized
Conjugation	Unconjugated
MW	16389 MW
UniProt ID	O15392
Storage	Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Alternative names	Baculoviral IAP repeat-containing protein 5;Apopto Read more...
Note	For research use only
Application notes	Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Expiration Date	12 months from date of receipt.

WB analysis of Survivin using anti-Survivin antibody.Lane 1:293T cell.

WB analysis of Survivin using anti-Survivin antibody.Lane 1:rat pc-12 Cell;2:human K562 Cell;3:mouse thymus Tissue.

IHC analysis of Survivin using anti-Survivin antibody.Survivin was detected in paraffin-embedded section of human intestinal cancer tissues.