BIRC5 Antibody

• Catalog Number: orb1240054
• Category: Antibodies
• Description: BIRC5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Anti-Survivin antibody (orb1240054) was raised against a peptide corresponding to 12 amino acids near the amino terminus of human Survivin. The immunogen is located within the first 50 amino acids of Survivin.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-5μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL; IHC: 5 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples; Immunofluorescence and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 17kDObserved: 17 kD
• Target: BIRC5
• UniProt ID: O15392
• NCBI: NP_001159
• Storage: Survivin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Survivin Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Survivin Antibody: API4, EPR-1, API4, IAP4, Baculo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human MOLT4 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: Survivin orb1240054 (A: 1 µg/mL and B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry Validation of Survivin in Jurkat Cells. Immunocytochemical analysis of Jurkat cells using anti-Survivin antibody (orb1240054) at 5 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of Survivin in Mouse Brain Tissue. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Survivin antibody (orb1240054) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry Validation of Survivin in Jurkat Cells. Immunocytochemical analysis of Jurkat cells using anti-Survivin antibody (orb1240054) at 5 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Survivin orb1240054 (5 µg/mL), Survivin orb1240039 (4 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.