BIRC5 Antibody

• Catalog Number: orb1240039
• Category: Antibodies
• Description: BIRC5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-Survivin antibody (orb1240039) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Survivin. The immunogen is located within the last 50 amino acids of Survivin.
• Concentration: 1 mg/ml
• Dilution range: WB: 1-5 μg/mL; ICC: 10 μg/mL; IF: 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 17kDObserved: 17 kD
• Target: BIRC5
• UniProt ID: O15392
• NCBI: NP_001159
• Storage: Survivin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Survivin Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Survivin Antibody: API4, EPR-1, API4, IAP4, Baculo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human MOLT4 Cell Lysate with the absence (A) or presence (B) of blocking peptide. Loading: 15 µg of lysates per lane. Antibodies: Survivin orb1240039 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Survivin orb1240054 (5 µg/mL), Survivin orb1240039 (4 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Survivin orb1240039 (4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry Validation of Survivin in MOLT4 Cells. Immunocytochemical analysis of MOLT4 cells using anti-Survivin antibody (orb1240039) at 10 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of Survivin in MOLT4 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed MOLT4 cells labeling Survivin with orb1240039 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).

Slot Blot Validation of Survivin in Hepatocellular Carcinoma (HCC) Patients (Zhang et al., 2007). Slot blot analysis of Survivin recombinant protein expression with anti-Survivin antibodies (orb1240039) in the four representative HCC sera. Survivin (Lane 7) was detected in the fourth HCC serum (E), but not in the normal human serum (A) and the other three HCC sera (B-D). Phosphate bufferedsaline (PBS) (Lanes 9 and 10) was used as a negative control.

Western Blot Validation of Survivin with Recombinant Protein (Megliorino et al., 2005). Lane 1: A 28 kDa peptide that corresponded to the size of ORF of survivin was detected in SDS-PAGE with Coomassie blue staining. Lane 2: WB analysis of Survivin recombinant protein expression with anti-Survivin antibodies (orb1240039).

Western Blot Validation of Survivin with Recombinant Protein in Sera from Cancer Patients (Megliorino et al., 2005). WB analysis of Survivin recombinant protein expression with anti-Survivin antibodies (orb1240039) in six cancer sera. 6 x His-tagged Survivin recombinant protein (Lanes 4-9) was strongly detected at 28kD, but not in the normal human serum (Lane 3).