Survivin antibody

• Catalog Number: orb1294349
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Survivin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Dilution range: Western Blot 1:2000-1:3000 Immunofluorescence 1:400-1:800 Immunohistochemistry (Paraffin) 1:100-1:300
• Conjugation: Unconjugated
• Target: Survivin / BIRC5
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Survivin/BIRC5 antibody at 1/2000 dilution. Lysates at 40 μg per lane. This blot was produced using a 15% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294349 overnight at 4°C.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Anti-Survivin/BIRC5 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling Survivin/BIRC5 with orb1294349 at 1/150.

Immunohistochemical analysis of paraffin embedded Human Tonsil cancer tissue labeling Survivin/BIRC5 with orb1294349 at 1/150.