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Catalog Number | orb758997 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to Spike protein (RBD) |
Species/Host | Camelus |
Clonality | Monoclonal |
Clone Number | Sb#14 |
Tested applications | ELISA |
Reactivity | Virus |
Isotype | Camelus IgG-Fc Fusion |
Immunogen | This clone was originally isolated in a form of a synthetic nanobody (sybody) via a 'target swap' selection procedure against RBD-vYFP using ribosomal display and against RBD-Fc fusion during phage display rounds. |
Purity | Purified |
Conjugation | Unconjugated |
Target | Spike protein (RBD) |
UniProt ID | P0DTC2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | Receptor Binding Domain; SARS CoV 2 S glycoprotein Read more... |
Note | For research use only |
Application notes | This antibody is recommended for detection of SARS CoV 2 or 2019-nCoV. Its high specificity to the novel coronavirus SARS-Cov-2 was confirmed via ELISA testing (Walter et al., 2020). Furthermore, grating-coupled interferometry demonstrated that this antibody binds the receptor binding domain (RBD) of the spike protein with high affinity (Walter et al., 2020). Subsequent testing showed that this clone exhibits exceptionally strong inhibition of binding of SARS-Cov-2 RBD to hACE2, which is the receptor for the virus (signal of RBD association with hACE2 decreased over 90%) (Walter et al., 2020). |
Expiration Date | 12 months from date of receipt. |
ELISA, IF, IHC, WB | |
Virus | |
Rabbit | |
Polyclonal | |
Unconjugated |
Unconjugated | |
90% | |
101.9 kDa | |
SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (orb640170) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1). |
FC, NeA | |
Virus | |
Rabbit | |
Recombinant | |
Unconjugated |
FA | |
Other | |
Monoclonal | |
Unconjugated |
ELISA | |
Virus | |
Camelus | |
Recombinant | |
Unconjugated |
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