SARS-CoV-2 (COVID-19) S1 RBD Single Domain Antibody [T4P5-H12]

• Catalog Number: orb1274197
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) S1 RBD Single Domain Antibody [T4P5-H12]
• Target: S
• Clonality: Recombinant
• Isotype: sdAb
• Conjugation: Unconjugated
• Reactivity: Virus
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) S1 RBD Antibody [T4P5-H12] Antibody is supplied in PBS.
• Purification: SARS-CoV-2 (COVID-19) S1 RBD Antibody [T4P5-H12] is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
• Immunogen: SARS-CoV-2 S protein RBD containing C-terminal His Tag. The protein was expressed in human 293 cells (HEK293). It contains amino acids Arg 319 - Lys 537.
• Tested applications: ELISA
• Antibody Type: Primary Antibody
• Clone Number: T4P5-H12
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Alternative names: SARS-CoV-2 (COVID-19) Spike S1 Antibody: Severe ac
• Note: For research use only
• NCBI: QHD43424

ELISA Validation with SARS-CoV-2 (COVID-19) Spike Trimer Proteins. Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, orb1274197. A direct ELISA was performed using SARS-CoV-2 Spike trimer recombinant proteins of WT, Omicron and Delta variants as coating antigen at 1 μg/mL and the anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibody (orb1274197) as the capture antibody, following by anti-cMyc-tag antibody (orb1239964) at 1 μg/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.4 ng/mL to 900 ng/mL. SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, orb1274197 can detect spike trimer protein at 1.5 ng/mL.

ELISA Validation with SARS-CoV-2 (COVID-19) Spike RBD, S1 and ECD Proteins Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, orb1274197. A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, orb1231588; S1, orb1227612; ECD, orb1231547) as coating antigen at 1 μg/mL and the anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibody (orb1274197) as the capture antibody, following by anti-cMyc-tag antibody (orb1239964) at 1 μg/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, orb1274197 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.