SARS-CoV-2 (COVID-19) S1 RBD Single Domain Antibody [T4P5-H12]

• Catalog Number: orb1274197
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) S1 RBD Single Domain Antibody [T4P5-H12]
• Species/Host: Camelus
• Clonality: Recombinant
• Clone Number: T4P5-H12
• Tested applications: ELISA
• Reactivity: Virus
• Isotype: sdAb
• Immunogen: SARS-CoV-2 S protein RBD containing C-terminal His Tag. The protein was expressed in human 293 cells (HEK293). It contains amino acids Arg 319 - Lys 537.
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S
• NCBI: QHD43424
• Storage: SARS-CoV-2°COVID-19) S1 RBD antibody should be stored in working aliquots at -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) S1 RBD Antibody [T4P5-H12] Antibody is supplied in PBS.
• Alternative names: SARS-CoV-2 (COVID-19) Spike S1 Antibody: Severe ac
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA Validation with SARS-CoV-2 (COVID-19) Spike Trimer Proteins. Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, orb1274197. A direct ELISA was performed using SARS-CoV-2 Spike trimer recombinant proteins of WT, Omicron and Delta variants as coating antigen at 1 μg/mL and the anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibody (orb1274197) as the capture antibody, following by anti-cMyc-tag antibody (orb1239964) at 1 μg/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.4 ng/mL to 900 ng/mL. SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, orb1274197 can detect spike trimer protein at 1.5 ng/mL.

ELISA Validation with SARS-CoV-2 (COVID-19) Spike RBD, S1 and ECD Proteins Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, orb1274197. A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, orb1231588; S1, orb1227612; ECD, orb1231547) as coating antigen at 1 μg/mL and the anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibody (orb1274197) as the capture antibody, following by anti-cMyc-tag antibody (orb1239964) at 1 μg/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, orb1274197 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.