SMAD9 Antibody

• Catalog Number: orb1151837
• Category: Antibodies
• Description: Mouse monoclonal antibody to SMAD9
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-SMAD9-2F4
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human SMAD9 protein was used as the immunogen for the SMAD9 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This SMAD9 antibody is available for research use only.
• UniProt ID: O15198
• Storage: Aliquot the SMAD9 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the SMAD9 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells using SMAD9 antibody (green, clone PCRP-SMAD9-2F4) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with SMAD9 antibody (blue, clone PCRP-SMAD9-2F4) and isotype control (red).

SDS-PAGE analysis of purified, BSA-free SMAD9 antibody (PCRP-SMAD9-2F4) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using SMAD9 antibody (clone PCRP-SMAD9-2F4). These results demonstrate the foremost specificity of the PCRP-SMAD9-2F4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.