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Catalog Number | orb1953526 |
---|---|
Category | Proteins |
Description | NSP8 is a nonstructural protein of coronavirus. NSP8 acts as a primase in RNA synthesis. NSP8 and NSP7 are essential co-factors of NSP12 (the catalytic subunit with RNA-dependent RNA polymerase activity) that can remarkably stimulate RdRp activity. The nsp12-nsp7-nsp8 subcomplex is defined as the minimal core component for mediating coronavirus RNA synthesis. SARS-CoV-2 NSP8 Protein is expressed in E. coli expression system. The predicted molecular weight is 22.04 kDa and the accession number is YP_009725304.1. |
Tag | Tag Free |
Purity | 98.00% |
MW | 22.04 kDa (predicted); 21.93 kDa (reducing conditions) |
UniProt ID | YP_009725304.1 |
Expression System | E. coli |
Biological Origin | SARS-CoV-2 |
Biological Activity | NSP8 is a nonstructural protein of coronavirus. NSP8 acts as a primase in RNA synthesis. NSP8 and NSP7 are essential co-factors of NSP12 (the catalytic subunit with RNA-dependent RNA polymerase activity) that can remarkably stimulate RdRp activity. The nsp12-nsp7-nsp8 subcomplex is defined as the minimal core component for mediating coronavirus RNA synthesis. SARS-CoV-2 NSP8 Protein is expressed in E. coli expression system. The predicted molecular weight is 22.04 kDa and the accession number is YP_009725304.1. |
Expression Region | A DNA sequence encoding the SARS-CoV-2 NSP8 (YP_009725304.1) (Ala1-Gln198) was expressed. Predicted N terminal: Glu |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
WB | |
> 95% as determined by SDS-PAGE. | |
22-25 kDa | |
E. coli |
GSH-Agarose (0.95) | |
Mono-Isotopic Mass: 48, 673.74 daltons Average Mass: 48, 705.25 daltons | |
bacteria |
Amylose Resin (0.95) | |
Mono-Isotopic Mass: 66, 078.48 daltons Average Mass: 66, 120.00 daltons | |
bacteria |
> 85% as determined by SDS-PAGE quantitative densitometry by Coomassie Blue Staining. | |
24.18kDa | |
E. coli |