SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein

• Catalog Number: orb1231613
• Category: Proteins
• Description: SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein
• Tested applications: ELISA, WB
• Tag: His tag at C-terminus
• Dilution range: This recombinant protein can be used for WB, ELISA. For research use only.
• Form/Appearance: Lyophilized
• Purity: > 90% as determined by SDS-PAGE; > 95% by HPLC.
• MW: 75.8 kDa
• Target: S
• UniProt ID: P0DTC2
• Protein Sequence: Val16-Arg685
• Source: HEK293 cells
• Biological Activity: Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human ACE2 at 2 g/mL (100L/well ) can bind Recombinant nCoV-S1, The EC50 of nCoV-S1 is 0.25-0.45 μg/mL
• NCBI: YP_009724390.1
• Storage: Store the lyophilized protein at -20°C to -20°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week
• Buffer/Preservatives: PBS, pH 7.4
• Alternative names: S1 protein, Spike glycoprotein subunit 1, S glycop
• Note: For research use only
• Application notes: This recombinant protein can be used for WB, ELISA. For research use only.
• Expiration Date: 6 months from date of receipt.

Immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 at 2μg/mL (100 μL/well) can bind recombinant human ACE2 with a linear range of 0.5-8.7 ng/mL.

The purity of SARS-CoV-2 (COVID-19) Spike S1 recombinant protein was greater than 95% as determined by SEC-HPLC.

SARS-CoV-2 (COVID-19) spike S1 recombinant protein on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.