SARS-CoV-2 (COVID-19) Spike 156-157EF Antibody

• Catalog Number: orb1239051
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Spike 156-157EF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 Spike 156-157EF antibody (orb1239051) was raised against a peptide corresponding to 13 amino acids near the amino terminal domain of SARS-CoV-2 Spike protein. The immunogen is located within 130-180 amino acids of SARS-CoV-2 Spike protein.
• Concentration: 1 mg/mL
• Dilution range: WB: 1 μg/mL. Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike 156-157EF antibody specifically does not detect SARS-CoV-2 Delta Variant (B.1.617.2) Spike S1 protein, but detects Spike S1 protein of WT and other variants by ELISA. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S
• UniProt ID: P0DTC2
• NCBI: QHD43416
• Storage: SARS-CoV-2 Spike 156-157EF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 Spike P26S antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SARS-CoV-2 Spike 156-157EF antibody: Severe acute
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry Validation of Spike in Delta Variant COVID-19 Patient Lung Tissue. Immunohistochemical analysis of paraffin-embedded patient lung tissue using anti-Spike 156-157EF antibody (orb1239051) at 0.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in the WT COVID-19 patient lung, but not in Delta variant COVID-19 patient lung.

SARS-Cov-2 Spike 156-157EF Antibodies Specifically Do Not Recognize Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and mu variant (B.1.621), 1 µg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, orb1239051, 1 µg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr.

SARS-Cov-2 Spike 156-157EF Antibodies Specifically Do Not Recognize Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 µg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike 1566-157EF antibody, orb1239051, 1 µg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr.

WB Validation of of Spike 156-157EF Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Spike 156-157EF antibody, orb1239051, 1 µg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubated at RT for 1 hr. SARS-CoV-2 Spike 156-157EF antibody (orb1239051) detects spike S1 protein of WT, but not Delta variant.

WB Validation of of Spike 156-157EF Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Spike 156-157EF antibody, orb1239051, 1 µg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubated at RT for 1 hr. SARS-CoV-2 Spike 156-157EF antibody (orb1239051) detects spike S1 protein of WT, but not Delta variant.