SARS-CoV-2 (COVID-19) Spike L452R Antibody (Delta Variant)

• Catalog Number: orb1239036
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Spike L452R Antibody (Delta Variant)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 Spike L452R (Delta Variant) antibody (orb1239036) was raised against a peptide corresponding to 9 amino acids in the receptor binding domain of SARS-CoV-2 Delta variant (B.1.617.2) Spike protein. The immunogen is located within 430-480 amino acids of SARS-CoV-2 Delta variant (B.1.617.2) Spike protein.
• Concentration: 1 mg/mL
• Dilution range: WB: 1 μg/mL. Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike L452R (Delta Variant) antibody specifically detects SARS-CoV-2 Delta Variant (B.1.617.2) Spike S1 protein, but not SARS-CoV-2 WT and other variant Spike S1 proteins by ELISA. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S
• UniProt ID: P0DTC2
• NCBI: QHD43416
• Storage: SARS-CoV-2 Spike Delta Variant antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 Spike Delta Variant antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SARS-CoV-2 Spike 156-157EF antibody: Severe acute
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

SARS-Cov-2 Spike L452R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and mu variant (B.1.621), 1 µg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, orb1239036, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubated at RT for 1 hr.

SARS-Cov-2 Spike L452R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 µg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, orb1239036, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubated at RT for 1 hr.

WB Validation of of Delta Variant Spike Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, orb1239036, 1 µg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubated at RT for 1 hr. SARS-CoV-2 Delta Variant Spike antibody (orb1239036) specifically detects Delta variant spike S1 protein, but not WT spike S1 protein (orb1231589).

WB Validation of of Delta Variant Spike Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, orb1239036, 1 µg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubated at RT for 1 hr. SARS-CoV-2 Delta Variant Spike antibody (orb1239036) specifically detects Delta variant spike S1 protein, but not WT spike S1 protein (orb1231589).