S100A4 Antibody / FSP1

• Catalog Number: orb750307
• Category: Antibodies
• Description: Mouse monoclonal antibody against S100A4 conjugated to Biotin
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Biotin
• Reactivity: Human, Mouse
• Buffer/Preservatives: 0.1 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: A portion of amino acids 1-200 from the human protein was used as the immunogen for the FSP1 antibody.
• UniProt ID: P26447
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 5ul/million cells,Western blot: 2-4ug/ml,Immunofluorescence: 2-4ug/ml at RT,Immunohistochemistry (FFPE): 2-4ug/ml at RT
• Application notes: Optimal dilution of the FSP1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: S100A4/1481
• Formula: 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Store the FSP1 antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term).
• Hazard Information: This FSP1 antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human placenta with biotinylated FSP1 antibody (clone S100A4/1481). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC testing of FFPE human placenta with biotinylated FSP1 antibody (clone S100A4/1481). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using S100A4/FSP1 antibody (clone S100A4/1481). These results demonstrate the foremost specificity of the S100A4/1481 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free FSP1 antibody as confirmation of integrity and purity.