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S100A4 Antibody / FSP1

Catalog Number: orb2640282

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2640282
CategoryAntibodies
DescriptionS100A4/FSP1 (Fibroblast specific protein 1) belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including p53, p63, non-muscle myosin IIA, a6b4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
Species/HostMouse
ClonalityMonoclonal
Clone NumberS100A4/1482
Tested applicationsFACS, IF, IHC-P, WB
ReactivityHuman, Mouse
IsotypeMouse IgG1, kappa
ImmunogenHuman recombinant S100A4 protein was used as the immunogen for this S100A4 antibody.
Antibody TypePrimary Antibody
Dilution rangeFlow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis S100A4 antibody is available for research use only.
UniProt IDP26447
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesThe optimal dilution of the S100A4 antibody for each application should be determined by the researcher.
Expiration Date12 months from date of receipt.
S100A4 Antibody / FSP1

Western blot testing of human 1) HeLa, 2) A549 and 3) A431 cell lysate with S100A4 antibody (clone S100A4/1482). Predicted molecular weight ~12 kDa.

S100A4 Antibody / FSP1

IHC testing of FFPE human placenta with S100A4 antibody (clone S100A4/1482). Required HIER: steam sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling.

S100A4 Antibody / FSP1

Western blot testing of human HeLa cell lysate with S100A4 antibody (clone S100A4/1482). Predicted molecular weight ~12 kDa.

S100A4 Antibody / FSP1

Flow cytometry testing of human T98G cells with S100A4 antibody (clone S100A4/1482); Red = isotype control, Blue = S100A4 antibody.

S100A4 Antibody / FSP1

Flow cytometry testing of human A549 cells with S100A4 antibody (clone S100A4/1482); Red = isotype control, Blue = S100A4 antibody.

S100A4 Antibody / FSP1

SDS-PAGE analysis of purified, BSA-free S100A4 antibody (clone S100A4/1482) as confirmation of integrity and purity.

S100A4 Antibody / FSP1

IF/ICC testing of fixed and permeabilized human HeLa cells with FSP1 antibody (clone S100A4/1482, green) and Reddot nuclear stain (red).

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    FACS,  IF,  IHC-P,  WB

    Human, Mouse

    Mouse

    Monoclonal

    Unconjugated

    20 μg
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    FACS,  IF,  IHC-P,  WB

    Human, Mouse

    Mouse

    Monoclonal

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    100 μg
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    FACS,  IF,  IHC-P,  WB

    Human, Mouse

    Mouse

    Monoclonal

    Unconjugated

    20 μg
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    FACS,  IF,  IHC-P,  WB

    Human, Mouse

    Mouse

    Recombinant

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    20 μg
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    FACS,  IF,  IHC-P,  WB

    Human, Mouse

    Mouse

    Recombinant

    Unconjugated

    100 μg