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S100A4 Antibody / FSP1

Catalog Number: orb2643761

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2643761
CategoryAntibodies
DescriptionS100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
Species/HostMouse
ClonalityRecombinant
Clone NumberrS100A4/1481
Tested applicationsFACS, IF, IHC-P, WB
ReactivityHuman, Mouse
IsotypeMouse IgG1, kappa
ImmunogenA recombinant human partial protein (amino acids 1-200) was used as the immunogen for the recombinant FSP1 antibody.
Antibody TypePrimary Antibody
Dilution rangeFlow cytometry: 1-2ug/million cells in 0.1ml,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Hazard InformationThis recombinant FSP1 antibody is available for research use only.
UniProt IDP26447
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
S100A4 Antibody / FSP1

Flow cytometry testing of permeabilized human A549 cells with FSP1 antibody (clone rS100A4/1481); Red=isotype control, Blue= FSP1 antibody.

S100A4 Antibody / FSP1

IHC testing of FFPE human placenta with FSP1 antibody (clone rS100A4/1481). Required HIER: steam sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling.

S100A4 Antibody / FSP1

IHC testing of FFPE human placenta with FSP1 antibody (clone rS100A4/1481). Required HIER: steam sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling.

S100A4 Antibody / FSP1

IF/ICC testing of fixed and permeabilized human A549 cells with FSP1 antibody (clone rS100A4/1481, green) and Reddot nuclear stain (red).

S100A4 Antibody / FSP1

Western blot testing of human PANC-1 cell lysate with FSP1 antibody. Predicted molecular weight ~12 kDa.

S100A4 Antibody / FSP1

SDS-PAGE analysis of purified, BSA-free recombinant FSP1 antibody (clone rS100A4/1481) as confirmation of integrity and purity.

  • S100A4 Antibody / FSP1 [orb750070]

    FACS,  IF,  IHC-P,  WB

    Human, Mouse

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • S100A4 Antibody / FSP1 [orb2640280]

    FACS,  IF,  IHC-P,  WB

    Human, Mouse

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • S100A4 Antibody / FSP1 [orb750071]

    FACS,  IF,  IHC-P,  WB

    Human, Mouse

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • S100A4 Antibody / FSP1 [orb2640282]

    FACS,  IF,  IHC-P,  WB

    Human, Mouse

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • S100A4 Antibody / FSP1 [orb639913]

    FACS,  IF,  IHC-P,  WB

    Human, Mouse

    Mouse

    Recombinant

    Unconjugated

    20 μg