Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody

• Catalog Number: orb1806450
• Category: Antibodies
• Description: Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR284L
• Tested applications: WB
• Reactivity: Human, Mouse
• Immunogen: Between 225 and 275
• Antibody Type: Primary Antibody
• Concentration: 500 µg/ml
• Dilution range: WB: 1:1000
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PVRL1/NECTIN1
• UniProt ID: Q15223
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: PVRL1; nectin-1; OFC7; poliovirus receptor-like 1;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human PVRL1/NECTIN1 by western blot. Samples: Whole cell lysate (10 µg) from MCF-10A, SNB-75, HaCaT, MCF-7, and RT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1806450) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse PVRL1/NECTIN1 by western blot. Samples: Whole cell lysate (50 µg) from ND7/23, C6, RAW 264.7, J774A1, and AML12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1806450) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.