Rabbit anti-eIF3A/eIF3S10 Antibody

• Catalog Number: orb1527406
• Category: Antibodies
• Description: eIF3A/eIF3S10 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 1 and 50
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: eIF3A/eIF3S10
• UniProt ID: Q14152
• NCBI: NP_003741.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: p180; eIF3-theta; eukaryotic translation initiatio
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse eIF3A/EIF3S10 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-eIF3A/EIF3S10 antibody orb1527406 used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human eIF3A/EIF3S10 by western blot of immunoprecipitates (h). Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF3A/EIF3S10 antibody orb1527406 used for IP at 3 µg/mg lysate.