Rabbit anti-eIF3A/eIF3S10 Antibody

• Catalog Number: orb1527404
• Category: Antibodies
• Description: eIF3A/eIF3S10 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 875 and 925
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 or Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: eIF3A/eIF3S10
• UniProt ID: Q14152
• NCBI: NP_003741.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: p180; eIF3-theta; eukaryotic translation initiatio
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse eIF3A/eIF3S10 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF3A/eIF3S10 antibody orb1527404 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human eIF3A/eIF3S10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3A/eIF3S10 antibody orb1527404 used for IP at 6 µg per reaction.

Detection of human eIF3A/EIF3S10 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-eIF3A/EIF3S10 (Cat. No. orb1527404) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB

Detection of mouse eIF3A/EIF3S10 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-eIF3A/EIF3S10 (Cat. No. orb1527404) used at a dilution of 1: 200 (1µg/ml). Detection: DAB