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Catalog Number | orb1530024 |
---|---|
Category | Antibodies |
Description | CTCF Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 677 and C-terminus |
Antibody Type | Primary Antibody |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | CTCF |
UniProt ID | P49711 |
NCBI | NP_006556.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | CTCFL paralog; MRD21; CCCTC-binding factor (zinc f Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of mouse CTCF by western blot.Samples: Whole cell lysate from CT26, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-CTCF antibody at 0.1 µg/ml.
Detection of human CTCF by western blot.Samples: Whole cell lysate from HEK293T, MCF-7, Hep-G2, A-549, and RKO cells. Antibody: Affinity purified rabbit anti-CTCF antibody at 0.1 µg/ml.
Detection of human CTCF by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-CTCF antibody.
FC, ICC, IHC, IP, SW-Size, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, ICC, IHC, IP, SW-Size, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
ChIP, IF, IH, WB | |
Human, Mouse, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |