UNG enzyme

SKU: orb423166

Description

UNG enzyme

Research Area

Epigenetics & Chromatin

Images & Validation

• Application Notes:
  Unit Definition: 1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37°C. Inactivation: Inactivated by heating at 95°C for 10min. Enzyme activity is partially restored at temperatures lower than 55°C. Reaction Conditions: 1X UNG Reaction Buffer, incubate at 37°C.UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites. 10X UNG Reaction Buffer: 200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA

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Key Properties

• Source: Escherichia Coli strain that carries the UNG gene from E.coli

Storage & Handling

• Storage: Stability: Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored below -18°C. Please prevent freeze-thaw cycles
• Form/Appearance: Sterile Filtered colorless solution.
• Buffer/Preservatives: UNG solution in 10mM Tris-HCl (pH-7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml rAlbumin and 50% glycerol.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Uracil DNA Glycosilase, Uracil DNA Glycosylase, UNG.