SYBR Green Premix Taq HS qPCR Kit (UNG Plus)

SKU: orb3229859

Description

This is a 2X premix type kit for qPCR using SYBR Green I chimeric fluorescence method. The reaction solution is very simple to prepare by adding primers, template and RNase free water. It is a 2X premix type reagent, and the reaction solution is easy to prepare, requiring only the addition of primers, template and RNase free water. Biorbyt Taq The HS DNA polymerase system enables highly sensitive Real Time PCR reactions for accurate quantification and detection of target genes. The dUTP/UNG Anti-Contamination System is also introduced in this product, which replaces dTTP with dUTP in the PCR reaction, utilizing the fact that UNG enzyme can selectively hydrolyze dU-containing DNA strands without affecting dU-free DNA strands, and removes dU-containing contaminating templates that are introduced during the preparation of the PCR reaction system, thus preventing the generation of false-positive results of the PCR and improving the accuracy of experimental results. This can effectively prevent the generation of PCR false-positive results and improve the accuracy of experimental results.

Images & Validation

• Application Notes:
  ①This product is a 2X premixed solution, premixed with SYBR Green I. The reaction solution is very simple to prepare, only need to add primers, templates and RNase free water to carry out qPCR reaction. ② The product has been optimized for SYBR Green I concentration and PCR reaction system, featuring high amplification efficiency and strong amplification specificity. The dUTP/UNG Contamination Prevention System has been introduced to remove contamination of dU-containing PCR products, thereby preventing false-positive PCR results and improving the accuracy of experimental results.

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Storage & Handling

• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only