2X Taq HS PCR Master Mix (UNG and Dye Plus)

SKU: orb3229832

Description

The dUTP/UNG Anti-Contamination System is introduced in this product, which replaces dTTP with dUTP in the PCR reaction, and utilizes UNG enzyme to selectively hydrolyze dU-containing DNA strands without affecting dU-free DNA strands to remove dU-containing contaminating templates introduced in the preparation of the PCR reaction system, thus effectively preventing the generation of false-positive results of the PCR and enhancing the accuracy of the results. This can effectively prevent the generation of false-positive PCR results and improve the accuracy of the results. This product also contains an additive that can inhibit the production of chemical substances at room temperature. Biorbyt Taq An active monoclonal antibody that enables Hot Start PCR and effectively inhibits primer dimer formation and non-specific amplification. The PCR product obtained with this product has an A base at the 3’ end and can be cloned directly into T vectors. At the same time, this product is a 2X PCR reaction premix, only need to add primers, templates and water to the premix to carry out the PCR reaction. At the same time, the electrophoresis indicator is added into the product, the solution of the product shows purple color, and can be used for agarose gel electrophoresis directly after the PCR reaction. It is easy to operate, minimizes human error, and results can be obtained in a short period of time.

Images & Validation

• Application Notes:
  1. dUTP/UNG is a contamination prevention system that removes contamination of dU-containing PCR products to prevent false-positive PCR results and improve the accuracy of the results. 2、This product adopts the superior performance of the Biorbyt Taq HS and optimized reaction system can effectively inhibit non-specific amplification. 3、This product is a 2X premix, the reaction solution is very simple to prepare, only need to add primers, templates and water to carry out the PCR reaction. 4、This product contains electrophoresis indicator, which can be used for electrophoresis directly after the PCR reaction, and there is only one indicator band in electrophoresis, and the indicator band shows light purple-red color.

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Storage & Handling

• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only