PGP9.5 antibody

Catalog Number	orb33715
Category	Antibodies
Description	Rabbit polyclonal antibody to PGP9.5
Species/Host	Rabbit
Clonality	Polyclonal
Clone Number	RB7379
Tested applications	FC, IF, IHC-P, WB
Predicted Reactivity	Equine, Porcine
Reactivity	Human, Mouse, Rat
Isotype	Rabbit IgG
Immunogen	Synthetic Peptide
Dilution range	WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50, IF/ICC: 1:10-50
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Conjugation	Unconjugated
MW	24824
Target	UCHL1
UniProt ID	P09936
NCBI	NP_004172.2
Alternative names	anti-Epididymis luminal protein 117 antibody, anti
Note	For research use only
Application notes	Protein InformationName	UCHL1Function	Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Cellular Location	Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brainTissue Location	Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients
Expiration Date	12 months from date of receipt.

Immunohistochemical analysis of formalin-fixed paraffin embedded human cancer tissue using PGP9.5 antibody (Dilution at :1:200)

Immunofluorescense analysis of NCI-H460 cell lysate using PGP9.5 antibody

Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram) using PGP9.5 antibody