PGP9.5 antibody

• Catalog Number: orb1474209
• Category: Antibodies
• Description: Mouse monoclonal antibody to PGP9.5
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, WB
• Reactivity: Human
• Immunogen: Purified recombinant human UCHL1 / PGP9.5 protein fragments expressed in E.coli.
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100)
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Conjugation: Unconjugated
• Target: UCHL1
• Entrez: 7345
• UniProt ID: P09936
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: Ubiquitin carboxyl-terminal hydrolase isozyme L1;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PGP9.5 expression in U251MG (A), Raji (B), IMR32 (C) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 25 kD)

Immunofluorescent analysis of PGP9.5 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.