PFKFB3 Antibody

• Catalog Number: orb1256708
• Category: Antibodies
• Description: PFKFB3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human PFKFB3 (NP_004557.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:1000IHC: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 53kDa
• Target: PFKFB3
• UniProt ID: Q16875
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphata
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PFKFB3 antibody (orb1256708) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat ovary using PFKFB3 antibody (orb1256708) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using PFKFB3 antibody (orb1256708) at dilution of 1:100 (40x lens).