Phospho-PFKFB3/PFK2 (Ser467) Rabbit Polyclonal Antibody

SKU: orb6697

Description

Phospho-PFKFB3/PFK2 (Ser467) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Equine, Gallus, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human PFK2 around the phosphorylation site of Ser467 LA(p-S)PE
• Target: PFKFB3
• Molecular Weight: 60 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

6 phosphofructo 2 kinase/fructose 2, 6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2, 6 biphosphatase 3; 6PF 2 K/Fru 2, 6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2, 6 P2ase 3; fructose 6 phosphate, 2 kinase/fructose 2, 6 bisphosphatase; Inducible 6 phosphofructo 2 kinase/fructose 2, 6 bisphosphatase; iPFK 2; IPFK2; PFK/FBPase 3; PFK-2; PFK2; Renal carcinoma antigen NY REN 56; uPFK 2; PFKFB3; F263_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Human glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PFKFB3 PFK2 (Ser467)) Polyclonal Antibody, Unconjugated (orb6697) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PFKFB3/PFK2 (Ser467)) Polyclonal Antibody, Unconjugated (orb6697) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PFKFB3/PFK2 (Ser467)) Polyclonal Antibody, Unconjugated (orb6697) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-PFKFB3/PFK2 (Ser467) (orb6697) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD.

Quick Database Links

Gene Symbol

PFKFB3

UniProt

Q16875