PD1 antibody

• Catalog Number: orb758881
• Category: Antibodies
• Description: Rabbit monoclonal antibody to PD1
• Species/Host: Hamster
• Clonality: Monoclonal
• Clone Number: J43
• Tested applications: ELISA, FC, IHC, IP
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: This antibody was raised by immunising Armenian hamsters with B12 cells, a PD-1 cDNA transfectant of BHK cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: PD-1
• UniProt ID: Q02242
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD279; PD1; PD 1; Programmed cell death protein 1;
• Note: For research use only
• Application notes: The specificity of this antibody has been confirmed in ELISA analysis, using PD-1 extracellular domain fusion proteins (Agata et al, 1996). Additionally, in flow cytometric analysis, this antibody reacts with PD-1 cDNA-transfected BHK and CHO cells, but not with parental BHK and CHO cells, as well as reacting with lymphocytes from PD-1 cDNA transgenic mice (Agata et al, 1996). This antibody has been used to immunoprecipitate PD-1 from lysates of PD-1 cDNA-transfected BHK and CHO cells (Agata et al, 1996), in flow cytometric quantification of CD4+PD-1+ T cells in murine spleens (Kasagi et al, 2010), and in immunohistochemical analysis of acetone-fixed murine spinal cord and brain tissue sections (Salama et al, 2003). This antibody displays diverse effects in different mouse models of disease. When administered to NZB/W F1 mice, a model of lupus-like nephritis, this antibody has been shown to delay the onset of nephritis and prolong survival, through the depletion of PD-1+ T cells (Kasagi et al, 2010). Antibody-treated NZB/W F1 mice displayed decreased numbers of PD-1+ T cells, and this antibody was confirmed to trigger complement-dependent cytotoxicity in PD-1+ T cells in vitro (Kasagi et al, 2010). Conversely, administration to experimental allergic encephalitis (EAE) and NOD diabetes mice exacerbated disease, through its neutralising activity (Salama et al, 2003; Ansari et al, 2003); this antibody has been shown in vitro to inhibit binding of both PD-L1-Ig and PD-L2-Ig to PD-1 transfected BHK cells (Ansari et al, 2003).
• Expiration Date: 12 months from date of receipt.