PDCD1 Antibody

• Catalog Number: orb1239734
• Category: Antibodies
• Description: PDCD1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 10B3
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: PD-1 antibody was raised against the extracellular domain of human PD-1.
• Concentration: 1 mg/mL
• Dilution range: PD-1 antibody can be used for detection of PD-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Flow cytometry at 0.1 μg/ml.Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 32 kDa Observed: 38 kDa
• Target: PDCD1
• UniProt ID: Q15116
• NCBI: NP_005009
• Storage: PD-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PD-1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Alternative names: PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD
• Note: For research use only
• Application notes: PD-1 antibody can be used for detection of PD-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Flow cytometry at 0.1 μg/ml.Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 [10B3] antibody at 10 μg/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 10 μg/mL.

(A) Immunohistochemistry of PD-1 in human spleen tissue with PD-1 [10B3] antibody at 10 μg/mL. (B) Immunohistochemistry in human spleen tissue with control mouse IgG staining at 10 μg/mL.

Immunofluorescence of PD-1 in human spleen tissue with PD-1 [10B3] antibody at 10 μg/mL. Green: PD1 Antibody [10B3] (orb1239734) Blue: DAPI staining

Flow cytometry analysis of PD-1 transfected 293 cells using PD-1 [10B3] antibody at 1 μg/ml. Blue: untransfected cells, Yellow: PD-1 transfected cells.

(A) Immunohistochemistry of PD-1 in human breast cancer tissue with PD-1 [10B3] antibody at 10 μg/mL. (B) Immunohistochemistry in human breast cancer tissue with control mouse IgG staining at 10 μg/mL.

Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA with orb1239745, orb1239769, orb1239763, orb1239738, and orb1239734 abs at decreasing concentrations.