PDCD1 Antibody

• Catalog Number: orb1239732
• Category: Antibodies
• Description: PDCD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-PD-1 antibody (orb1239732) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human PD-1. The immunogen is located within amino acids 210-260 of PD-1.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-4 μg/mL; IHC: 2.5-5 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Human PD-1 has 1 isoform (288aa, 32kD). Mouse PD-1 has 1 isoform 288aa, 32kD) and Rat PD-1 also has one isoform (287aa, 32kD).
• Target: PDCD1
• UniProt ID: Q15116
• NCBI: Q15116
• Storage: PD-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PD-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

KO Validation in HeLa Cells. Loading: 10 µg of HeLa WT cell lysates or PD-1 KO cell lysates. Antibodies: PD-1, orb1239732 (4 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

KD Validation in HeLa Cells. Loading: 10 µg of HeLa WT cell lysates or PD-1 KD cell lysates. Antibodies: PD-1, orb1239732 (4 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PD-1 orb1239732 (4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in THP-1 Cell Lysate in the (A) absence and (B) presence of blocking peptide. Loading: 15 µg of lysates per lane. Antibodies: PD-1, orb1239732 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Thymus Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: PD-1 orb1239732 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of PD-1 in Human Tonsil Tissue. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD-1 antibody (orb1239732) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of PD-1 in Human Tonsil Tissue. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD-1 antibody (orb1239732) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of PD-1 in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-PD-1 antibody (orb1239732) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.