PDCD1 Antibody

• Catalog Number: orb1239763
• Category: Antibodies
• Description: PDCD1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 7H6
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: PD-1 antibody was raised against the extracellular domain of human PD-1.
• Concentration: 1 mg/mL
• Dilution range: PD-1 antibody can be used for detection of PD-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 32 kDa Observed: 38 kDa
• Target: PDCD1
• UniProt ID: Q15116
• NCBI: NP_005009
• Storage: PD-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PD-1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Alternative names: PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD
• Note: For research use only
• Application notes: PD-1 antibody can be used for detection of PD-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PD-1 in transfected 293 cell lysate with PD-1 antibody at 1 μg/mL.

Immunohistochemistry of PD-1 in (A) human tonsil tissue, (B) human lymph node tissue, and (C) human spleen tissue with PD-1 antibody at 5 μg/mL. (D) Immunohistochemistry in human tonsil tissue with control mouse IgG staining at 5 μg/mL.

Immunohistochemistry of PD-1 in (A) human breast cancer tissue and (B) human normal breast tissue with PD-1 antibody at 5 μg/mL.

Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 5 μg/mL.

Immunofluorescence of PD-1 in transfected 293 cells with PD-1 antibody at 5 μg/mL. Lower left: Immunofluorescence in transfected 293 cells with control mouse IgG antibody at 5 μg/mL. Red: PD1 Antibody [7H6] (orb1239763) Blue: DAPI staining

Western blot analysis of PD-1 in overexpressing 293 cells using orb1239745, orb1239769, and orb1239763 antibody at 1, 0.5, and 0.25 μg/ml, respectively.

Flow cytometry analysis of PD-1 overexpressing 293 cells using orb1239763 at 1 μg/ml. Blue: untransfected cells, Yellow: PD-1 transfected cells.

Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA with orb1239745, orb1239769, orb1239763, orb1239738, and orb1239734 abs at decreasing concentrations.