PCGF3 Antibody

• Catalog Number: orb1823460
• Category: Antibodies
• Description: Polycomb group (PcG) proteins form multiprotein complexes that regulate expression patterns of developmental and cell proliferation genes. Several members of the PcG contain ring finger domains and are identified as a subclass of RING finger proteins. The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteinerich domain that is able to bind two zinc atoms. Proteins that contain the RING-type zinc finger conserved domain are generally involved in the ubiquitination pathway of protein degradation. PCGF3 (polycomb group ring finger 3), also known as RNF3, DONG1 or RNF3A, is a 242 amino acid transcriptional regulator that is encoded by a gene located on human chromosome 4, which encodes nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. PCGF3 exists as two isoforms produced by alternative splicing events.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human protein was used as the immunogen for the PCGF3 antibody.
• UniProt ID: Q3KNV8
• Tested applications: FACS, IF, IHC-P
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Antibody Type: Primary Antibody
• Clone Number: PCRP-PCGF3-1D5
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This PCGF3 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human prostate tissue with PCGF3 antibody (clone PCRP-PCGF3-1D5) HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free PCGF3 antibody (clone PCRP-PCGF3-1D5) as confirmation of integrity and purity.

Flow cytometry testing of PFA-fixed human HeLa cells with PCGF3 antibody (clone PCRP-PCGF3-1D5) followed by goat anti-mouse IgG-CF488 (blue); Red = unstained cells.

Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using PCGF3 antibody (clone PCRP-PCGF3-1D5). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.