p27 Antibody / p27Kip1

• Catalog Number: orb2635987
• Category: Antibodies
• Description: p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-b induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: KIP1/9168R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Recombinant full-length human CDKN1B/p27Kip1 protein was used as the immunogen for the recombinant p27 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This recombinant p27 antibody is available for research use only.
• UniProt ID: P46527
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the recombinant p27 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human prostate tissue. Strong nuclear staining observed using recombinant p27 antibody at 2 ug/ml. HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant p27 antibody (clone KIP1/9168R) as confirmation of integrity and purity.