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Ornithine Decarboxylase Antibody / ODC1

Catalog Number: orb385793

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb385793
CategoryAntibodies
DescriptionOrnithine Decarboxylase (ODC) is the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [RefSeq]
Species/HostMouse
ClonalityMonoclonal
Clone NumberORN255-1
Tested applicationsFACS, IHC-P, WB
ReactivityHuman
IsotypeMouse IgG1, kappa
ImmunogenRecombinant human protein was used as the immunogen for this ODC antibody.
Antibody TypePrimary Antibody
Dilution rangeFlow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis ODC antibody is available for research use only.
Entrez4953
StorageStore the ODC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesTitering of the ODC antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
Ornithine Decarboxylase Antibody / ODC1

Western blot testing of ODC antibody and placenta lysate. Predicted molecular weight ~51 kDa.

Ornithine Decarboxylase Antibody / ODC1

IHC testing of FFPE human prostate carcinoma and ODC antibody (clone ORN255-1). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.

Ornithine Decarboxylase Antibody / ODC1

Flow analysis of permeablized PC3 cells using ODC antibody (green, clone ORN255-1) and isotype control (black).

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    FACS,  IF,  IHC-P,  WB

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    FACS,  IF,  IHC-P,  WB

    Human, Mouse, Rat

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    FACS,  IF,  IHC-P,  WB

    Human, Rat

    Mouse

    Monoclonal

    Unconjugated

    20 μg