NELFE Antibody

• Catalog Number: orb1527566
• Category: Antibodies
• Description: NELFE Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 75 and 125
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 5 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NELFE
• UniProt ID: P18615
• NCBI: NP_002895.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: negative elongation factor polypeptide E; RNA-bind
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human NELFE by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NELFE antibody orb1527566 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. NELFE was also immunoprecipitated by a previous lot of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).