NELFE antibody

• Catalog Number: orb341214
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NELFE
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human
• Immunogen: Recombinant full length protein of human NELF-E
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:50-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: NELFE
• Entrez: 7936
• UniProt ID: P18615
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: RD; RDBP; Negative elongation factor E; NELF-E; RN
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NELF-E expression in SW620 (A), Hela (B), mouse liver (C), mouse testis (D) whole cell lysates. (Predicted band size: 40; 43 kD; Observed band size: 43 kD)

Immunofluorescent analysis of NELF-E staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.