NEK2 Antibody

• Catalog Number: orb1262771
• Category: Antibodies
• Description: NEK2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This NEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-426 amino acids from the Central region of human NEK2.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:100For IF starting dilution is: 1:25For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 52 kDa
• Target: NEK2
• UniProt ID: P51955
• NCBI: P51955
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Serine/threonine-protein kinase Nek2, HSPK 21, Nev
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:100For IF starting dilution is: 1:25For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. brain section using NEK2 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Fluorescent image of U251 cells stained with hNEK2-C410. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded R. brain section using NEK2 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. brain section using NEK2 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from 293T, Hela, Jurkat, K562, MCF-7, and mouse NIH/3T3 cell line (from left to right), using NEK2 Antibody (C410) at 1:1000 at each lane.

Western blot analysis of anti-NEK2 Antibody in HL60 cell line lysates (35 ug/lane)