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Catalog Number | orb80075 |
---|---|
Category | Proteins |
Description | Mouse IL13 protein |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 95% as determined by SDS-PAGE. |
Conjugation | Unconjugated |
Target | Mouse IL13 |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Interleukin 13 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | The sequence of the first five N-terminal amino acids was determined and found to be Met-Pro-Val-Pro-Arg. |
Biological Origin | E.coli |
Biological Activity | The ED50 range=2-6 ng/ml, determined by the dose dependent prolifiration of TF-1 cells. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | The protein (1mg/ml) was lyophilized with no additives. |
Alternative names | Allergic rhinitis protein, ALRH protein, BHR 1 pro Read more... |
Note | For research use only |
Application notes | < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.69 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of IL-13 as Reference Standard. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Mouse IL13 protein
> 97% as determined by SDS-PAGE and HPLC. | |
12.3 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
13.1 kDa | |
Mammalian cell |
Greater than 95% as determined by SDS-PAGE. | |
12.7 kDa | |
Mammalian cell |
Greater than 95% as determined by SDS-PAGE. | |
11.7 kDa | |
E.coli |
Unconjugated | |
90% | |
14.2 kDa | |
Mouse IL-13, His Tag (orb1176550) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Phe 131 (Accession # P20109-1). |