mCherry antibody

• Catalog Number: orb11618
• Category: Antibodies
• Description: Goat polyclonal antibody to mCherry (Cherry fluorescent protein).
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: IEM, IF, IHC-Fr, IHC-P, WB
• Reactivity: Other
• Isotype: IgG
• Immunogen: Purified recombinant peptide produced in E. coli.
• Concentration: 1 mg/ml
• Dilution range: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
• Form/Appearance: Polyclonal antibody supplied as a 200 or 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
• Conjugation: Unconjugated
• Target: mCherry
• Storage: Store at -20°C for long-term storage. Store at 2-8°C for up to one month.
• Buffer/Preservatives: PBS, 20% glycerol and 0.05% sodium azide
• Alternative names: Cherry fluorescent protein; dsRed, red fluorescent
• Note: For research use only
• Application notes: The antibody solution should be gently mixed before use.
• Expiration Date: 12 months from date of receipt.

References

Bárbara Adem 1 2, Nuno Bastos 1 2, Carolina F Ruivo 1, Sara Sousa-Alves 1, Carolina Dias 1 3, Patrícia F Vieira 1 3, Inês A Batista 1 2, Bruno Cavadas 1, Dieter Saur 4 5, José C Machado 1 6 7, Dawen Cai 8 9 10, Sonia A Melo Exosomes define a local and systemic communication network in healthy pancreas and pancreatic ductal adenocarcinoma Nat Commun, 15, 1496 (2024)

PubMed: 38383468

Applications

WB

Reactivity

Human

Olga A Balashova # 1, Alexios A Panoutsopoulos # 2, Olesya Visina 2, Jacob Selhub 3, Paul S Knoepfler 4, Laura N Borodinsky Noncanonical function of folate through folate receptor 1 during neural tube formation Nat Commun, 15(1):, 1642 (2024)

PubMed: 38388461

Applications

IHC

Reactivity

Human

Allison M Kirk 1, Jeremy Chase Crawford 1, Ching-Heng Chou 1, Cliff Guy 1, Kirti Pandey 2, Tanya Kozlik 3, Ravi K Shah 3, Shanzou Chung 2, Phuong Nguyen 4, Xiaoyu Zhang 5, Jin Wang 6, Matthew Bell 4, Robert C Mettelman 1, E Kaitlynn Allen 1, Mikhail V Pogorelyy 1, Hyunjin Kim 1, Anastasia A Minervina 1, Walid Awad 1, Resha Bajracharya 7, Toni White 5, Donald Long Jr 8, Brittney Gordon 9, Michelle Morrison 10, Evan S Glazer 11, Andrew J Murphy 12, Yixing Jiang 13, Elizabeth A Fitzpatrick 6, Mark Yarchoan 14, Praveen Sethupathy 8, Nathan P Croft 2, Anthony W Purcell 2, Sara M Federico 15, Elizabeth Stewart 16, Stephen Gottschalk 4, Anthony E Zamora 3, Christopher DeRenzo 4, Scott E Strome 17, Paul G Thomas DNAJB1-PRKACA fusion neoantigens elicit rare endogenous T cell responses that potentiate cell therapy for fibrolamellar carcinoma Cell Rep Med, 5, 101469 (2024)

PubMed: 38508137

Applications

IF

Reactivity

Mouse

Talley MJ, Nardini D, Ehrman LA, Lu QR, Waclaw RR Distinct requirements for Tcf3 and Tcf12 during oligodendrocyte development in the mouse telencephalon Neural Dev., 18(1), 5 (2023)

PubMed: 37684687

Zhou, Tian et al. Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice Nat Commun, 13, 6233 (2022)

PubMed: 36280666

Huang, Yubin et al. Repopulated microglia are solely derived from the proliferation of residual microglia after acute depletion Nat Neurosci, 21, 530-540 (2018)

PubMed: 29472620

Gong, ChenZi et al. Human spinal GABA neurons alleviate spasticity and improve locomotion in rats with spinal cord injury Cell Rep, 34, 108889 (2021)

PubMed: 33761348

Jonscher, Ernst et al. Two COWP-like cysteine rich proteins from Eimeria nieschulzi (coccidia, apicomplexa) are expressed during sporulation and involved in the sporocyst wall formation Parasit Vectors, 8, 395 (2015)

PubMed: 26209229

Stincic, Todd L. et al. Arcuate and Preoptic Kisspeptin neurons exhibit differential projections to hypothalamic nuclei and exert opposite postsynaptic effects on hypothalamic paraventricular and dorsomedial nuclei in the female mouse eNeuro, (2021)

PubMed: 34281980

Ramesh, Girish et al. A short isoform of STIM1 confers frequency-dependent synaptic enhancement Cell Rep, 34, 108844 (2021)

PubMed: 33730587

Li, Fei et al. Murine leukemia virus Gag localizes to the uropod of migrating primary lymphocytes J Virol, 88, 10541-10555 (2014)

PubMed: 24965475

Western blot analysis of transfected 293 HEK with mCherry cell lysate using mCherry antibody

Immunohistochemical analysis of mouse mammary tumor cell line using mCherry goat antibody

Confocal immunofluorescence analysis of COS-7 transfected with mCherry-EEA1 using mCherry antibody