MBD2 antibody

• Catalog Number: orb19388
• Category: Antibodies
• Description: Goat polyclonal antibody to MBD2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 2.5 μg/ml
• Conjugation: Unconjugated
• MW: 43.3; 31.7
• Target: MBD2 (isoform 1)
• Entrez: 8932
• Protein Sequence: RNDPLNQNKGKPDLN
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti MBD2 antibody, anti methyl-CpG binding domain
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Colon shows nuclear stianing of enterocytes. Recommended concentration, 2-4ug/ml.WB: Approx 48-50kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Biol. 2006 Oct;26(19):7224-35.; PMID: 16980624). Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Jurkat cell lysate using MBD2 antibody.

Immunohistochemical staining of Human Colon using MBD2 antibody.

Immunohistochemical staining of Human Adrenal Gland using MBD2 antibody.

Immunohistochemical staining of Human Skin using MBD2 antibody.