MBD2 antibody

• Catalog Number: orb1473647
• Category: Antibodies
• Description: Mouse monoclonal antibody to MBD2
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: Recombinant fusion protein of human MBD2. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200)
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MBD2
• Entrez: 8932
• UniProt ID: Q9UBB5
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Methyl-CpG-binding domain protein 2; Demethylase;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MBD2 expression in K562 (A), MCF7 (B), Jurkat (C) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 43; 32 kD)

Immunohistochemical analysis of MBD2 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.