MBD2 Rabbit Polyclonal Antibody

SKU: orb329663

Description

Rabbit polyclonal antibody to MBD2

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MBD2
• Target: MBD2
• Protein Sequence: Synthetic peptide located within the following region: RAHPGGGRCCPEQEEGESAAGGSGAGGDSAIEQGGQGSALAPSPVSGVRR
• Molecular Weight: 32kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti DKFZp586O0821 antibody, anti DMTase antibody, anti NY-CO-41 antibody

Lanes: Lane 1: 15 ug WT mouse ES lysate, Lane 2: 15 ug MBD2 KO mouse ES lysate, Primary Antibody Dilution: 1:1000, Secondary Antibody: Goat anti-rabbit-HRP, Secondary Antibody Dilution: 1:2500, Gene Name: MBD2 a.

Rabbit Anti-MBD2 Antibody, Catalog Number: orb329663, Formalin Fixed Paraffin Embedded Tissue: Human Adult liver, Observed Staining: Nuclear, Primary Antibody Concentration: 1:100, Secondary Antibody: Donkey anti-Rabbit-Cy2/3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5 – 2.0 sec, Protocol located in Reviews and Data.

WB Suggested Anti-MBD2 Antibody Titration: 0.2-1 ug/mL, ELISA Titer: 1:12500, Positive Control: 721_B cell lysate, MBD2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.

Quick Database Links

Gene Symbol

MBD2

UniProt

Q9UBB5

RefSeq (Protein):NP_056647