MAPK3 antibody

• Catalog Number: orb18996
• Category: Antibodies
• Description: Goat polyclonal antibody to MAPK3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Human
• Dilution range: ELISA: 1:4000, WB: 0.1-1 μg/ml, IHC-P: 10ug/ml
• Conjugation: Unconjugated
• MW: 43.1; 40.1; 38.3
• Target: MAPK3/ERK1
• Entrez: 5595
• Protein Sequence: GGEPRRTEGVGP
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti MAPK3 antibody, anti ERK1 antibody, anti mito
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 45kDa band observed in Human Brain lysates, Kidney fibroblast HEK293 lysates and epidermoid fibroblast A431 lysates (calculated MW of 43.1kDa according to NP_002737.1). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18996 (0.3ug/ml) staining of 293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing ERK1 (RC204196) and probed with orb18996 (mock transfection in second lane), tested by Origene