MAPK3 antibody

• Catalog Number: orb129600
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MAPK3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human ERK1 (NP_002737.2).
• Concentration: 1 mg/ml
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:10-100
• Conjugation: Unconjugated
• MW: 40kDa
• UniProt ID: P27361
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Alternative names: anti MAPK3 antibody, anti ERK1 antibody, anti HS44
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

He, Wen et al. p21-Activated Kinase 4 Signaling Promotes Japanese Encephalitis Virus-Mediated Inflammation in Astrocytes Front Cell Infect Microbiol, 7, 271 (2017)

PubMed: 28680855

Immunohistochemistry analysis of paraffin-embedded rat lung using [KD Validated] ERK1 Rabbit pAb (orb129600) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human kidney using [KD Validated] ERK1 Rabbit pAb (orb129600) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse heart using [KD Validated] ERK1 Rabbit pAb (orb129600) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Western blot analysis of lysates from wild type (WT) and ERK1 knockdown (KD) 293T cells, using [KD Validated] ERK1 Rabbit pAb (orb129600) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western blot analysis of various lysates using [KD Validated] ERK1 Rabbit pAb (orb129600) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.