MAPK1/MAPK3 Antibody

• Catalog Number: orb1258906
• Category: Antibodies
• Description: MAPK1/MAPK3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant Protein of human ERK1 / ERK2
• Concentration: batch dependent
• Dilution range: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 42kDa/44kDa
• Target: MAPK1/MAPK3
• UniProt ID: P27361, P28482
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: ERK, ERT1, MAPK 1, MAPK2, ERK2
• Note: For research use only
• Application notes: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ERK1/ERK2 antibody (orb1258906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunofluorescence analysis of C6 cells using ERK1/ERK2 antibody (orb1258906) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ERK1/ERK2 antibody (orb1258906) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using ERK1/ERK2 antibody (orb1258906) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using ERK1/ERK2 Polyclonal Antibody (orb1258906) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using ERK1/ERK2 Polyclonal Antibody (orb1258906) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using ERK1/ERK2 Polyclonal Antibody (orb1258906) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.